An azine based sensor for selective detection of Cu2+ ions and its copper complex for sensing of phosphate ions in physiological conditions and in living cells.

Title An azine based sensor for selective detection of Cu2+ ions and its copper complex for sensing of phosphate ions in physiological conditions and in living cells.
Authors K. Tiwari; S. Kumar; V. Kumar; J. Kaur; S. Arora; R.Kumar Mahajan
Journal Spectrochim Acta A Mol Biomol Spectrosc
DOI 10.1016/j.saa.2017.09.072
Abstract

A simple and cost effective unsymmetrical azine based Schiff base, 5-diethylamino-2-[(2-hydroxy-benzylidene)hydrazonomethyl]-phenol (1) was synthesized which selectively detect Cu2+ ions in the presence of other competitive ions through "naked eye" in physiological conditions (EtOH-buffer (1:1, v/v, HEPES 10mM, pH=7.4)). The presence of Cu2+ induce color change from light yellow green to yellow with the appearance of a new band at 450nm in UV-Vis spectra of Schiff base 1. The fluorescence of Schiff base 1 (10?M) was quenched completely in the presence of 2.7 equiv. of Cu2+ ions. Sub-micromolar limit of detection (LOD=3.4×10-7M), efficient Stern-Volmer quenching constant (KSV=1.8×105Lmol-1) and strong binding constant (log Kb=5.92) has been determined with the help of fluorescence titration profile. Further, 1-Cu2+ complex was employed for the detection of phosphate ions (PO43-, HPO42- and H2PO4-) at micromolar concentrations in EtOH-buffer of pH7.4 based on fluorescence recovery due to the binding of Cu2+ with phosphate ions. Solubility at low concentration in aqueous medium, longer excitation (406nm) and emission wavelength (537nm), and biocompatibility of Schiff base 1 formulates its use in live cell imaging.

Citation K. Tiwari; S. Kumar; V. Kumar; J. Kaur; S. Arora; R.Kumar Mahajan.An azine based sensor for selective detection of Cu2+ ions and its copper complex for sensing of phosphate ions in physiological conditions and in living cells.. Spectrochim Acta A Mol Biomol Spectrosc. 2018;191:1626. doi:10.1016/j.saa.2017.09.072

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